Lunch & Learn


L1: Latest FIB Sample Preparation and HR-STEM Developments

Date: May 22nd (Wednesday), 12:30pm to 1:30pm

Location: UBC LSC 1410

Organizer: SFR / Thermo Fischer Scientific

Maximum: 30 participants


With demand for TEM sample preparation at an all-time high, electron microscopists and researchers require sample generation to take much less than a day to prepare and corrected S/TEM requires ultra-thin (<30 nm) specimens with minimal damage.   New advances in FIB technology and automation are ready to tackle this challenge by preparing high quality, ultra-thin specimens in less than an hour.  Furthermore, STEM is being used to investigate a wider variety of material types than ever before with an aim to extract more information as efficiently as possible.  Advances in STEM technology and techniques allow sub-Å imaging of all elements in the periodic table with the accelerating voltage required by the sample whether high or low.  These and other developments are aimed at extending the level and quality of information that materials scientists can extract from the vast array of complex and often beam sensitive specimens that are being investigated in the electron microscope today and into the future and will be discussed during this contribution.


NOTE: L2 Lunch'N'Learn has been CANCELLED due to unforseen circumstances. 

L2: Olympus Tru technologies and their benefits to optical microscopy across all modalities

Date: May 22nd (Wednesday), 12:30pm to 1:30pm

Location: UBC LSC 1416

Organizer: Farid Jalali, Sr. Laser Scanning & Digital Imaging Systems Specialist, Olympus Canada

Sponsor: Olympus Canada


Optical microscopy continues to evolve at a rapid pace and the Olympus Tru technologies have been developed to benefit end users and their research across all platforms. Olympus’ Tru technologies include; (1) TruSpectral for laser scanning confocal microscopy, providing high light efficiency spectral imaging to multi-colour paradigms; (2) TruSight for cross modality GPU-enabled constrained iterative deconvolution, providing clearer and sharper images; (3) TruFocus for stability during longitudinal live cell imaging; and (4) TruResolutionfor multi-photon microscopy, providing dynamic, automated spherical aberration correction for brighter and sharper images at depth.  The Olympus Tru technologies will be discussed with an emphasis on defined research endpoints and imaging modalities that span the gamut from basic widefield fluorescence to super resolution to multi-photon microscopy.



L3: Managing (High) Tension: Atomic Resolution (S)TEM from 30kV to 300kV

Date: May 23rd (Thursday), 12:30pm to 1:30pm

Location: UBC LSC 1416

Organizer: Thomas Isabell

Sponsor: JEOL USA


Choosing the correct microscope high tension, or accelerating voltage is an important experimental consideration for TEM imaging and microanalysis. Higher accelerating voltages naturally lead to better spatial resolution for imaging, but will also lead to accelerated specimen damage. For microanalysis, lower accelerating voltages mean more beam spreading and worse spatial resolution, but also enhanced microanalysis cross sections for EDS and EELS. A new generation of aberration corrected microscopes gives the user the flexibility to operate over a wide range of high tension with unprecedented imaging and chemical spatial resolution. This means that on the same instrument, the voltage can be dialed in for a given experiment and readily changed as different experimental needs arise. The addition of a cold field emission gun, advanced aberration correctors and advanced detectors only enhances operation at both high and low voltages, further adding to instrument flexibility. 


L4: Fast Fluoresce lifetime imaging for Biosensors and imaging dynamic systems

Date: May 23rd (Thursday), 12:30pm to 1:30pm

Location: UBC LSC 1410

Sponsor: Leica Microsystems


The new Leica SP8 FALCON (FAst Lifetime CONtrast) is the future of functional imaging. It harnesses the power of fluorescence lifetime to investigate cellular physiology and explore dynamics in living cells.  This is an excellent technique for imaging stem cells in organoids based on differences in chromatin condensation or to image cancer cells in tissues because of differences in metabolites such as NAD, FAD Etc.
SP8 FALCON is the first truly integrated solution for Fluorescence Lifetime Imaging (FLIM) and delivers benchmark results at least 10x faster than conventional systems.
SP8 FALCON adds a new dimension of contrast to your imaging, opening the door to biosensing and tracking of interactions between proteins. FLIM information is now available for all modalities of the SP8 platform.
Now you can:
   •    Follow fast molecular interactions via FLIM-FRET (Förster Resonance Energy Transfer)
   •    Use biosensors to detect changes in metabolic state and microenvironment
   •    Apply lifetime contrast to separate multiple fluorophores
   •    Acquire fluorescence lifetime data with minimal training



L5: Seeing is Believing – New Horizons for In Situ Microscopy

Date: May 24th (Friday), 12:30pm to 1:30pm

Location: UBC LSC 1330

Organizer: Jordan Moering, Kate Marusak

Sponsor: Protochips


New innovations are transforming the Transmission Electron Microscope (TEM) from a simple high-resolution image acquisition tool into a nanoscale materials research and development laboratory. Researchers can now better understand material behavior by analyzing samples in real-world gas or liquid environments, at high temperature and with ultra-low noise electrochemical and electrical characterization techniques. With the new in situ tools from Protochips, materials research occurs in highly controlled environments at high resolution without sacrificing the analytical capabilities of the TEM such as EDS. Applications for these tools include heterogeneous catalysis, semiconductor fabrication, lithium-ion batteries, energy materials, memory storage, and more. In this presentation, we show some of the most recent in situ results and findings from leading researchers in fields spanning the entire scientific discipline from life science to material science.